题目内容
（请给出正确答案）
A、add DNA polymerase and nucleotides at 72℃
B、cool DNA to between 50℃ and 65℃
C、add primers
D、heat target DNA to 94℃
E、repeat the cycle of heating and cooling
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原文:Chinese communities enjoy a worldwide reputation as the “kingdom of cuisine”. The exquisite Chinese culinary art, regarded indisputably as one of the world’s finest culinary traditions, has prevailed all over the world. Since China has a vast territory, and the products, climate and living habits are quite different in each place, the flavor of food is quite different: southerners like light food, northerners prefer a heavily seasoned taste, Sichuan people like hot food, Shanxi people like sour food and so on.译文:中国享有“_________”之美誉,精致的_________盛行全球。中国菜不但花样多,而且具有色、香、味、形俱佳的特点。由于中国地域________,各地的物产、气候和生活习惯不同,因此人们的口味也各不相同:南方人口味________,北方人口味较重,四川人喜欢吃辣,山西人__________。
A、extraction of vector DNA, ligation of target DNA to vector DNA, restriction of target DNA
B、restriction of target DNA, ligation of cut (that is, restricted) target DNA to vector DNA which has already been cut, transformation of host cells
C、cutting target DNA, cutting vector DNA, amplifying target DNA
D、amplifying target DNA, ligating target DNA to cut vector DNA, homologous recombination of target and vector DNA
A、structure genes of some types of protein
B、structure genes of all types of protein
C、all genes
D、all genes’ introns and regulatory regions
A、genetic engineering
B、biotechnology
C、recombinant DNA
D、gel electrophoresis
E、gene probes
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