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提问人:网友fanren1985 发布时间:2022-01-07
[主观题]

After DNA extraction, agarose electrophoresis is required. What reagents need to be prepared or purchased?

简答题官方参考答案 (由简答题聘请的专业题库老师提供的解答)
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更多“After DNA extraction, agarose electrophoresis is required. What reagents need to be prepared or purc…”相关的问题
第1题
Cells have several ways of ensuring that their daughters remember what kind of cells they should be. How do differentiated cells maintain their identity?
A.One of the simplest and most important is through a positive feedback loop, where a master transcription regulator activates transcription of its own gene,in addition to that of other cell-typespecific genes. Each time a cell divides, the regulator is distributed to both daughter cells, where it continues to stimulate the positive feedback loop.?The continued stimulation ensures that the regulator will continue to be produced in subsequent cell generations.?Positive feedback is crucial for establishing the “self-sustaining” circuits of gene expression that allow a cell to commit to a particular fate—and then to transmit that decision to its progeny.

B.Although positive feedback loops are probably the most prevalent way of ensuring that daughter cells remember what kind of cells they are meant to be,there are other ways of reinforcing cell identity. One involves the methylation of DNA.In vertebrate cells, DNA methylation occurs on certain cytosine bases. This covalent modification generally turns off the affected genes by attracting proteins that bind to methylated cytosines and block gene transcription. DNA methylation patterns are passed on to progeny cells by the action of an enzyme that copies the methylation pattern on the parent DNA strand to the daughter DNA strand as it is synthesizeD

C.Another mechanism for inheriting gene expression patterns involves the modification of histones. When a cell replicates its DNA,each daughter double helix receives half of its parent’s histone proteins, which contain the covalent modifications that were present on the parent chromosom

E.Enzymes responsible for these modifications may bind to the parental histones and confer the same modifications to the new histones nearby. It has been proposed that this cycle of modification helps reestablish. the pattern of chromatin structure found in the parent chromosome

D.Because all of these cell-memory mechanisms transmit patterns of gene expression from parent to daughter cell without altering the actual nucleotide sequence of the DNA,they are considered to be forms of epigenetic inheritanc

E.These mechanisms, which work together, play an important part in maintaining patterns of gene expression, allowing transient signals from the environment to be remembered by our cells—a fact that has important implications for understanding how cells operate and how they malfunction in diseas

E.

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第2题
标准分子量DNA(DNAMarker)的主要作用是()。
A、没有具体作用

B、对照样品DNA

C、验证琼脂糖配置的浓度

D、检验成像的质量

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第3题
蛋白质合成抑制剂的作用机制( )。

A、抑制转肽酶

B、抑制转位酶

C、抑制核糖体70S亚基

D、与EFG-GTP结合

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第4题

命题考核:汽车合成修图自然风景篇/城市夜景篇(二选一) 内容要求:1. 老师提供车辆原档及部分背景素材,要求必须使用所提供车档案,可自行寻找背景素材(必须使用所提供背景素材不低于2张)2. 每张作品请附作品名,题目自拟 形式要求:画面主体明确,需有透视感及景深感,层次丰富,正确的元素关系,画面表现真实自然。 要求:1、尺寸大小:A4,72dpi,psd文件保留所有图层; 2、请将所有素材、完成效果设计成版面上传至答案框中,并简要写出你的构思。 3、将psd、jpg以及版面设计文件放置以自己名字命名的文件夹中,压缩进行上传。 参考效果:注意:汽车只能使用老师指定的素材,使用其它汽车素材一律0分计算。

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第5题
1.Find two kinds of restriction enzymes you are interested and draw the ends produced by the two restriction enzymes.
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第6题
2.Which sequence of nucleotides would you predict would appear more frequently in any genome? A) GACGTC or B) ACGT
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第7题
The Arabidopsis thaliana calcineurin B-like protein 1-15 (CBL1-15) protein sequences should be downloaded from the NCBI website. And you should export the sequences in the fast format and print it. The first three of the student number do CBL1, No. 4-6 doing CBL2, No. 7-9 doing CBL3, and so on.
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第8题
3.What type of enzyme might a genetic engineer use to protect a specific DNA site from restriction enzyme digestion?
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第9题

2. Please design three pairs of primers for this sequence. >NM_118791.4 Arabidopsis thaliana calcineurin B-like 3 (CBL3), mRNA AAATTATTTATTTTTTAAATTTAAAAAGTATAAAAACGCCAACAAACAAAAATAATTTGGTCTTTTTCTTAGCATATTTAAAATTTTGCAATATATTCATTCTGCTTATAGAAAATCGTGTCGACCTTTTTTCTCTCACTTACCCAATAAGAGCTTTTTTCGCGTTTTCCACCGCAACCACCGTCACCTTCAACCGCCGGAGCCGCCGCAAGGAAGGCTCATCATCCGCCGTGGCAATTTTTTGAGGTGTACAAAGCATTGCCTATTTGACGAACCAGGAAATGGTTAAAAGGCATGGAGAGTTTTGATTTACAAGATATATCATGTCGCAGTGCATAGACGGTTTCAAGCATGTATGCTCCTCTTTTTTCCGGTGTTTTGATATCGATATTTACAAACAATCTGGAGGCCTTGGTGACCCTGAACTTCTTGCGAGAGAGACAGTTTTTAGCGTGAGTGAAATAGAGGCTCTCTATGAGCTGTTTAAGAAAATCAGCAGTGCTGTGATTGATGATGGGCTAATAAACAAGGAAGAATTTCAGTTAGCCTTGTTCAAGACAAATAAAAAAGAGAGCTTGTTTGCAGACCGGGTATTCGATTTGTTTGACACAAAGCATAATGGAATACTGGGGTTTGAGGAGTTTGCGCGTGCTCTCTCTGTCTTTCATCCAAATGCTCCCATAGAAGACAAGATTGACTTTTCGTTTCAGCTATATGATCTTAAGCAGCAAGGTTTCATAGAGAGGCAAGAGGTGAAGCAAATGGTGGTGGCTACTCTTGCTGAGTCCGGCATGAACCTGTCAGACGAAATCATAGAGAGTATAATCGACAAGACATTTGAGGAAGCTGATACAAAACATGACGGGAGGATCGATAAGGAAGAGTGGAGAACCCTTGTTCTCAGGCATCCTTCCCTTCTAAAGAATATGACTCTCCAGTATCTTAAGGATATAACAACTACATTTCCGAGCTTTGTGTTCCACTCGCAGGTGGAAGATACCTGAGAAAAACAATAGCATATTAGCAGAGGAGACAATTTGGGAATTGTGTTTGTTTTCTGATTATGTATCATGTCTATTTGTTTCAATCTCTCTGAACATTTTCAAGATTTTGTTGTTACTTGTGTGAATTAAAAGTAAAGAAAACAAATCTGTATTTTTGCTACTCCTGATTTTGATTGCTGATTTTCTCCTCGCATCTGTAGATATACTACTAACCTTGAGACATGA

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